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Diphosphoinositol Elisa Test Equipment Polyphosphate Phosphohydrolase 3 Beta

Categories Human ELISA Kit
Place of Origin: China , Canada
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
brand: DLdevelop
certificate: ISO9001:2015, CE
size: 48T
Assay length: 1-4.5Hours
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    Diphosphoinositol Elisa Test Equipment Polyphosphate Phosphohydrolase 3 Beta

    Diphosphoinositol Elisa Test Equipment Polyphosphate Phosphohydrolase 3 Beta

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Diphosphoinositol Polyphosphate Phosphohydrolase 3-Beta (NUDT11) ELISA Kit
    Method:Competition
    Synonyms:

    hAps1; hAps1(Nudix motif 11); Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-beta; 3.6.1.60; Nucleoside diphosphate-linked moiety X motif 11(Nudix motif 11)

    Detection range:0.312-20ng/mL
    Reactivity:NUDT11
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-NUDT11-Hu (traditional)DLR-NUDT11-Hu (ready-to-use)
    Assay length1-2.5Hours1-2.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1.Overview:

    Other names:hAps1; hAps1(Nudix motif 11); Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-beta; 3.6.1.60; Nucleoside diphosphate-linked moiety X motif 11(Nudix motif 11)

    Function: Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate.

    Sequence:

    50 MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG
    100 GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVYVLTV
    150 TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT
    160 NGNSMAPSSP DSDP

    2.Features
    INTENDED USE
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NUDT11 in human serum, plasma, tissue homogenates or other biological fluids.
    DETECTION RANGE
    0.312-20ng/mL. The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
    SENSITIVITY
    The minimum detectable dose of NUDT11 is typically less than 0.101ng/mL.
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
    SPECIFICITY
    This assay has high sensitivity and excellent specificity for detection of NUDT11.
    No significant cross-reactivity or interference between NUDT11 and analogues was observed.
    You can reference link of the kit as following
    http://www.dldevelop.com/Research-reagent/dl-nudt11-hu.html
    http://www.dldevelop.com/uploadfile/data/DL-NUDT11-Hu.pdf
    Introduction
    <
    ItemStandardTest
    Description

    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human NUDT11 in serum, plasma, tissue homogenates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL

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