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Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China , Canada
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
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    Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

    Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

    0

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit
    Method:Sandwich
    Synonyms:

    CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8

    Detection range:1.56-100ng/mL
    Reactivity:HCAM
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-HCAM-Hu (traditional)DLR-HCAM-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    Introduction
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HCAM in human serum, plasma, tissue homogenates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL
    Detection Reagent B 1×120LDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1

    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

    After TMB substrate solution is added, only those wells that contain HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

    The concentration of HCAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Recovery

    Matrices listed below were spiked with certain level of recombinant HCAM and the recovery rates were calculated by comparing the measured value to the expected amount of HCAM in samples.

    MatrixRecovery range (%)Average(%)
    serum(n=5)80-10291
    EDTA plasma(n=5)81-10090
    heparin plasma(n=5)80-8984

    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample1:21:41:8


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