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Human14-3-3ProteinEpsilon(YWHAE)ELISAKit

Categories Human ELISA Kit
Place of Origin: China , Canada
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
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    Human14-3-3ProteinEpsilon(YWHAE)ELISAKit

    Human 14-3-3 Protein Epsilon (YWHAE) ELISA Kit

    0

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human 14-3-3 Protein Epsilon (YWHAE) ELISA Kit
    Method:Sandwich
    Synonyms:
    Detection range:78.1-5000pg/mL
    Reactivity:YWHAE
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-YWHAE-Hu (traditional)DLR-YWHAE-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1.Overview:

    Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Sequence:

    1020304050

    MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
    60708090100
    NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
    110120130140150
    LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
    160170180190200
    AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
    210220230240250
    DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV
    EDENQ
    2.Features
    INTENDED USE
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of YWHAE in human serum, plasma, tissue homogenates or other biological fluids.
    DETECTION RANGE
    78.1-5000pg/mL. The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78.1pg/mL.
    SENSITIVITY
    The minimum detectable dose of YWHAE is typically less than 32.9pg/mL.
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
    SPECIFICITY
    This assay has high sensitivity and excellent specificity for detection of YWHAE.
    No significant cross-reactivity or interference between YWHAE and analogues was observed.
    You can reference link of the kit as following
    http://www.dldevelop.com/uploadfile/data/DL-YWHAE-Hu.pdf
    http://www.dldevelop.com/Research-reagent/dl-ywhae-hu.html
    Introduction
    <
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of YWHAE in human serum, plasma, tissue homogenates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL
    Detection Reagent B 1×120LDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1
























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